[animal medical paper] the action that discusses prevention and cure to should stimulate asthma of the bandicoot below the condition

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[animal medical paper] the action that discusses prevention and cure to should stimulate asthma of the bandicoot below the condition

[paper summary] purpose: Law adjustment should stimulate the effect that discusses psychological element to come on to asthma and tone liver, the action mechanism of asthma of prevention and cure. Method: The albumen that use egg is added manacle should stimulate stimulation to build should stimulate a condition to finish asthmatic bandicoot model, use radiative immunity to analyse adsorption of immunity of law, enzymatic couplet law, former group of a cross, immunity is changed, the method such as smooth lens and electric lens, detect plasma of each groups of bandicoot is coriaceous element of ketone, leucocyte interpose 4, coriaceous hormone gets candy of interferon γ , sea horse body MRNA and adrenocorticotro of head issueing grave release hormone (Adrenocorticotropichormone Corticoliberin, CRH) electropositive nerve yuan, observe the change of lung and sea horse tectology. Result: Lung of scanty liver manage just can reduce level of serous and coriaceous ketone apparently, elevatory and serous interferon γ , reduce serous leucocyte interpose element 4, reduce electropositive nerve of CRH of the nucleus other ventricle issueing grave yuan, candy of area of sea horse CA3 is moved on the expression that coriaceous hormone gets body MRNA, reduce sea horse nerve yuan injury, can reduce the pathology change that should stimulate a condition to issue asthmatic bandicoot lung to organize apparently. Conclusion: Lung of scanty liver manage just should stimulate a condition to issue pituitary of the grave below asthmatic bandicoot through restraining the hyperfunction of function of adrenal coriaceous axis and adjust the function of immune system, have the effect of asthma of prevention and cure thereby.
[paper keyword] should stimulate law of lung of manage of scanty liver of; asthmatic;
Protective And Therapeutic Effects Of Shugan Lifei Recipe On Ratswith Asthma Under Stress And The Mechanisms
ABSTRACT
Objective: To Explore The Influence Ofpsychological Factor On Asthma, the Protective And Therapeuticeffects Of Shugan Lifei Recipe On Rats With Asthma Under Stress Andthe Mechanisms. Methods: Allergy And Stressinduced Asthma Modelswere Established In Rats By Giving Ovalbumin And Restrainingstress. Radioimmunoassay, enzyme Linked Immunosorbent Assay, insitu Hybridization And Immunohistrochemical Method Were Used Todetect Plasma Corticosterone, IL4, IFN γ , expression Ofglucocorticoid Receptor (GR) MRNA In Hippocampus Andadrenocorticotropic Hormone Corticoliberin (CRH) Positve Neuron Inhypothalamus. Optical And Electron Microscopes Were Used To Observethe Morphological Changes Of Pulmonary Hilar And Hippocampaltissues.Results: Shugan Lifei Recipe (SGLFR) Could Reduce Plasmacorticosterone, decrease CRH Positive Neurons In Paraventricularnucleus Of Hypothalamus, upregulate The Expression Of GR MRNA Inhippocampus And Reduce The Injury Of Hippocampal Neuron. SGLFR Hadcertain Inhibitive Effect On Hyperfunction OfhypothalamopituitaryadreNal Cortex (HPA) Axis In Rats With Asthmaunder Stress And Could Also Relieve The Pathological Changes Of Thepulmonary Hilar Tissue. Was Increasedwhile The Level Of Plasma IL4 Was Decreased In SGLFRtreated Group.Conclusion of γ of The Level Of Plasma IFN: The Mechanism Of SGLFR In Treating The Rats With Asthmaunder Stress Is Probably To Regulate The Hyperfunction Of HPA Axisand The Disorder Of Immuosystem.
KEY WORDSStress; Asthma; Dispersing Stagnatedliver Qi And Regulating Lung Qi
Bronchus asthma is disease of a kind of many pathogeny, hair ill mechanism is very complex, endanger human health badly. Its come on the effect that suffers biology, psychology and element of social tripartite face, there is main effect in the happening of mental psychology element in asthma, development and lapse to among them. Contemporary work and life rhythm are accelerated increasingly, competition is intense with each passing day, airframe is in nervous condition for a long time to fall, disturb the immune function of airframe through affecting then of nerve internal system, be caused thereby or aggravating asthma.
Medical early realises undesirable environment in or the happening of mental stimulation and body disease, development has close relationship. Liver advocate sparse release, move free air plane, stimulate in heart behoove there is conclusive effect in adjusting. Modern medicine thinks: Of any forms should stimulate can bring about nerve endocrine the function is disorder, create immune function obstacle, make airframe assumes easy feeling position to all sorts of infection sex diseases. Consider to make clear in great quantities: Liver advocate closely related the network of nerve endocrine immunity of sparse release function and modern medicine, the law that move liver has adjust the function with maladjusted function of nerve endocrine immunity. This experiment applies lung of scanty liver manage to just treat the asthmatic bandicoot that should stimulate a condition to fall, law adjustment should stimulate the effect that discusses psychological element to come on to asthma and tone liver, the action mechanism of asthma of prevention and cure.
1 material and method
1.1 experiments animal reachs a model to build in group
SD male bandicoot 56, the constitution is measured (190 ± 20) G, random cent is 7 groups: Regular set, asthmatic group, should stimulate group, asthma to should stimulate rice of a place of strategic importance of group, ground soft group, manage lung group and group of lung of scanty liver manage, every groups 8. Asthmatic group, asthma should stimulate rice of a place of strategic importance of group, ground soft group, manage lung group and scanty liver manage lobar group bandicoot gives abdominal cavity injects respectively antigen fluid (powder of the albumen that contain egg, hydroted alumina and destroy bacili of vivid whooping cough) 1ml is sent quick; Regular set and should stimulate group bandicoot to give abdominal cavity injects respectively physiological saline waiting for a quantity. Send quick hind since the 8th day, should stimulate group, asthma to should stimulate rice of a place of strategic importance of group, ground soft group, manage lung group and scanty liver manage lobar group bandicoot gives vertical of double hind legs is secured, manacle all right should stimulate stimulation, 4h/d, in all 3 weeks; Send quick hind since the 8th day, asthmatic group, asthma should stimulate rice of a place of strategic importance of group, ground soft group, manage lung group and scanty liver manage lobar group bandicoot is additional give respectively pulverization of 2% egg protein is inspiratory, 3min/d, in all 2 weeks.
1.2 medicaments reach formulary way
Manage lung square (Lifei Recipe, LFR) namely small Qing Longshang, fizzle out by hemp 9 G of 9 G of 9 G of 9 G of 9g, cassia twig, broil licorice 9 G, the root of Chinese wild ginger, dry ginger 9 G, the tuber of pinellia, the fruit of Chinese magnoliavine and composition of 9 G of root of herbaceous peony; Lung of scanty liver manage square (Shugan LifeiRecipe, SGLFR) namely small Qing Longshang closes 4 go against medicinal powder, fizzle out by hemp 9 G of 9 G of 9 G of 9 G of 9 G, cassia twig 9 G, broil licorice 9 G, the root of Chinese wild ginger, dry ginger 9 G, the tuber of pinellia, 9g of the fruit of Chinese magnoliavine, root of herbaceous peony, the root of Chinese thorowax and composition of solid 9 G of broil trifoliate orange. Manage lung just just uses the filtrate after water simmer in water is made with lung of scanty liver manage, make condense fluid of profess to convinced, contain crude drug 2.8 G/ml and 3.6g/ml respectively. Rice of ground a place of strategic importance is loose (0.75 Mg/ piece) mix at be being made up with physiological saline before fill stomach hang fluid, chroma is 0.1mg/ml. Send quick hind since the 8th day, soft group of rice of ground a place of strategic importance, manage lung group and bandicoot of group of lung of scanty liver manage give pine of rice of ground a place of strategic importance mixes lung of the 10 Ml/kg that hang fluid, manage respectively stomach of fill of 10ml/kg of fluid of square profess to convinced of 10ml/kg of fluid of square profess to convinced and lung of scanty liver manage; Regular set, should stimulate group, asthmatic group and asthma to should stimulate group bandicoot to give respectively stomach of fill of physiological saline waiting for a quantity. Give medicine 3 weeks in all.
1.3 animals processing reachs draw materials
Experiment the 4th on the weekend, inject with abdominal cavity of calm of 25% corvee labor formerly imposed on Xizang serfs anaesthetic bandicoot, draw-out abdomen aorta blood instantly infuse is fought coagulate in the canal, centrifugal 15min of 3 000 R/min, depart is serous, cent outfit is saved at - wait for in freezer of 70 low temperature measure. After celiac aorta exsanguinate instantly fill sheds bandicoot, every groups of bandicoot that choose fill to shed a success 3, examination of smooth lens of equipment of organization extraction lung and inspect across to organize equipment light to the head of tit body group of lens, report lens, immunity is changed and former a cross examination.
Of 1.4 serous and coriaceous ketone determine radiative immunity check checks serous and coriaceous ketone agent box, limited company of engineering of You Jingmei biology is offerred. The as emulative as the coriaceous ketone antigen that did not label union that the basis labels the principle of its particularity antibody undertakes detecting. The formation of complex matter measures antibody of antigen of coriaceous ketone of the 123I in reaction system the effect that the coriaceous ketone antigen that in getting sample, did not label measures, did not label the content of coriaceous ketone is jumped over in sample namely tall, criterion the form achievement of complex matter jumps over antibody of antigen of 123I coriaceous ketone little, through determining antigen antibody is compound the radioactivity of content, computation is relevant parameter, scale standard curve, numerate directly the content of coriaceous ketone in sample. Experimental measure presses operation of reagent box manual strictly.
Coriaceous hormone gets candy of 1.5 sea horse of body MRNA detect
Sea horse candy is coriaceous hormone accepts put oneself in another‘s position (Glucocorticoidreceptor, GR) MRNA former a cross reagent box, offer by limited company of engineering of doctoral mind being, use float the law detects. Take inspect across to be organized to the head of tit body, freeze continuously quickly all right section, detect measure undertakes strictly by reagent box manual, all be aseptic manipulation. Use IMS cell image to analyse systems analysis result.
Adrenocorticotro of 1.6 grave head releases hormone positive nerve yuan detect
Adrenocorticotro of head issueing grave releases hormone (Adrenocorticotropic Hormone Corticoliberin, CRH) electropositive nerve yuan use SABC immunity group to change reagent box to undertake detecting, reagent box is offerred by limited company of engineering of doctoral mind being. Take inspect across to be organized to the head of tit body, freeze continuously quickly all right section, detect measure undertakes strictly by reagent box manual. Use IMS cell image to analyse systems analysis result.
1.7 leucocyte interpose element 4 with interferon γ detect
Leucocyte interpose element 4 (Interleukin4, IL4) with interferon γ (Interferon γ , IFN γ ) box of reagent of enzymatic couplet immunity, all offer by limited company of engineering of brilliant beautiful live thing.
1.8 morphology observe
Morphology of smooth lens of 1.8.1 bandicoot sea horse observes
Take bandicoot to inspect across to be organized to the head of tit body, convention observes below lens solely.
1.8.2 bandicoot sea horse appears radio lens morphology to observe
According to rat head stereo fixed position pursues chart, take 1mm3 of tangent plane of shape of sea horse arrow constituent piece, groovy report lens issues observation.
1.8.3 bandicoot lung organizes observation of smooth lens morphology
Take organization of bandicoot lung door, convention observes below lens solely.
1.9 statistical methods
Use SPSS10.0 software to undertake statistical analysis. Metric data all is counted express with X ± S, use only factor variance analysis and Q to examine.
2 results
Square correspondence of lung of manage of 2.1 scanty liver stimulates a condition to issue asthmatic bandicoot plasma the influence of coriaceous ketone
Should stimulate group and regular set to compare, level of serous and coriaceous ketone is apparently elevatory (P<0.01) ; Asthma should stimulate group and asthmatic group to compare, the level also has serous and coriaceous ketone to lift apparently (P<0.01) ; Group of lung of scanty liver manage and manage lung group are compared, level of serous and coriaceous ketone is reduced apparently (P<0.01) . See a table 1.
Express 1
Coriaceous ketone level compares plasma of each groups of bandicoot (slightly)
Tab 1
Comparison Of Plasma Corticosterone Indifferent Groups
*P<0.05, **P<0.01, vs Normalcontrol Group; P<0.01, vs Allergyinduced Asthmamodel Group; P<0.01, vs Allergy And Stressinducedasthma Model Group; P<0.01, vs LFRtreated Group;P<0.05, p<0.01, vsdexamethasonetreated Group
Square correspondence of lung of manage of 2.2 scanty liver stimulates a condition to issue GR MRNA of asthmatic bandicoot sea horse and nerve of positive of CRH of head issueing grave yuan influence
Should stimulate group and regular set to compare, expression of sea horse GRmRNA decreases apparently (P<0.01) , electropositive nerve of CRH of head issueing grave yuan number increases apparently (P<0.01) ; Asthma should stimulate group and asthmatic group to compare, expression of sea horse GRmRNA decreases apparently (P<0.01) , electropositive nerve of CRH of head issueing grave yuan number increases apparently (P<0.01) ; Group of lung of scanty liver manage and manage lung group are compared, expression of sea horse GRmRNA increases apparently (P<0.01) , electropositive nerve of CRH of head issueing grave yuan amount decreases apparently (P<0.01) . See a table 2.
Square correspondence of lung of manage of 2.3 scanty liver surges the impact of configuration of asthmatic bandicoot sea horse below the condition
Smooth lens issues observation result: Cell of cone of sea horse of regular set bandicoot forms regulation, nucleolus is round and big, nucleolus clarity; Cell of cone of sea horse of asthmatic group bandicoot is arranged more regular, CA3 area sees dark nerve yuan; Should stimulate group and asthma to should stimulate cell of cone of group bandicoot sea horse to arrange irregular, in the cone nucleolus with CA3 visible and many area solid shrinks; Unusual change and asthma should stimulate soft group of rice of ground a place of strategic importance and cell of cone of sea horse of bandicoot of manage lung group group of basic close; Cell of cone of sea horse of bandicoot of group of lung of scanty liver manage is arranged more regular, in CA3 area nucleus solid shrinks nerve yuan decrease apparently.
Groovy transmission observes a result below electric lens: Form of nucleolus of cone of sea horse of regular set bandicoot is regular, nuclear film is smooth, nuclear different chromatin distributings even, line bead put oneself in another‘s position is substantial, ji forms regulation; Form of nucleolus of cone of sea horse of asthmatic group bandicoot is regular, but afterbirth oar is loose, occurrence vacuole of body of partial line bead, ji is arranged disorder; Should stimulate group and asthma to should stimulate configuration of nucleolus of cone of group bandicoot sea horse irregular, nuclear different chromatin is apparent grow in quantity, gather agglomerate, afterbirth oar is loose, dilate of body of most line bead, occurrence vacuole, Ji is arranged disorder; Configuration of cell of cone of sea horse of bandicoot of soft group of rice of ground a place of strategic importance is irregular, cell implement metamorphic; Oar of afterbirth of cell of cone of sea horse of bandicoot of manage lung group is loose, dilate of body of most line bead, occurrence vacuole; Form of nucleolus of cone of sea horse of bandicoot of group of lung of scanty liver manage is more regular, nuclear film is smooth, nuclear different chromatin distributings evener, afterbirth oar is loose, line bead put oneself in another‘s position is substantial but compact.

Square correspondence of lung of manage of 2.4 scanty liver stimulates a condition to play the effect of IL4 of asthmatic bandicoot plasma and IFN γ
Should stimulate group and regular set to compare, serous IL4 level is apparently elevatory (P<0.01) , IFN γ level is reduced apparently (P<0.01) ; Asthma should stimulate group and asthmatic group to compare, serous IL4 level is apparently elevatory (P<0.01) , IFN γ level is reduced apparently (P<0.01) ; Group of lung of scanty liver manage and manage lung group are compared, IL4 level does not have plasma to be reduced apparently, IFN γ level is apparently elevatory (P<0.05) . See a table 3.
Express 2
GR MRNA of sea horse of each groups of bandicoot and electropositive nerve of CRH of head issueing grave yuan quite (slightly)
Tab 2 Comparison Of Expression Of GR MRNA In Hippocampus Andnumber Of CRH Positive Neurons In Paraventricular Nucleus Ofhypothalamus In Different Groups
**P<0.01, vs Normal Control Group;P<0.01, vs Allergyinduced Asthma Model Group;P<0.01, vs Allergy And Stressinduced Asthma Modelgroup; P<0.01, vs LFRtreated Group;P<0.01, vs Dexamethasonetreated Group
Express 3
IL4 of plasma of each groups of bandicoot and IFN γ chroma are compared (slightly)
Tab 3
Indifferent Groups of γ of Comparison Of Plasma IL4 And IFN
**P<0.01, vs Normal Control Group;P<0.01, vs Allergyinduced Asthma Model Group;P<0.01, p<0.01, vs Allergy Andstressinduced Asthma Model Group; P<0.05, vsLFRtreated Group; P<0.05, vs Dexamethasonetreatedgroup
Square correspondence of lung of manage of 2.5 scanty liver stimulates a condition to issue asthmatic bandicoot lung to organize the influence of configuration
Bronchus of regular set bandicoot and alveolar structure are normal; Should stimulate group bandicoot alveolar wall is reached to have a few phlogistic cell soak all round bronchus; Asthmatic group and asthma should stimulate group bandicoot alveolar wall is reached to have soak of many phlogistic cell all round bronchus, bronchus is in charge of antrum stricture, epithelial fall off, there is secretion inside antrum; Rice of ground a place of strategic importance reachs alveolar wall to also cell of more phlogistic sex infiltrates all round bronchus of soft group bandicoot; Manage reachs soak of alveolar wall phlogistic cell to be reduced apparently all round lobar group and bronchus of bandicoot of group of lung of scanty liver manage.
3 discuss
Should be being stimulated is to point to all sorts of answering to airframe gets the pituitary of the following grave that when stimulating former stimulation, appears is adrenal coriaceous (HypothalamopituitaryadreNal, HPA) a series of nerve endocrine that excessive activation gives priority to the axis react. Measurable should stimulate the health that stimulates pair of human body and functional activity to rising to promote action, make airframe can get used to the change of environment of inside and outside; But the hyperfunction of HPA axis function that lasts for a long time, can bring about airframe to be in easy feeling condition to all sorts of infection sex elements, create the obstacle of immune function. When should be being stimulated, HPA axis activations, the nucleus other ventricle issueing grave (Paraventricularnucleus, PVN) cellule nerve yuan secrete CRH, the Xie Shi before CRH stimulates hypophysis puts an adrenocorticotro (Adrenocorticotropichormone, ACTH) , exciting adrenal coriaceous synthesis mixes ACTH secrete candy coriaceous hormone. Study the result makes clear originally: In should stimulate a condition to fall, asthmatic bandicoot plasma is coriaceous ketone level is apparently elevatory, the specification is manacled should stimulate stimulation to be able to be brought about should stimulate a condition to issue hyperfunction of function of axis of asthmatic bandicoot HPA, make level of serous and coriaceous ketone elevatory. Coriaceous ketone level and asthma should stimulate plasma of bandicoot of group of lung of scanty liver manage model group is reduced apparently quite, but coriaceous ketone level and asthma should stimulate plasma of bandicoot of manage lung group group do not have statistical difference quite, show lung of scanty liver manage to just can answer through restraining those who stimulate axis of the HPA below the condition is stimulant, reduce level of serous and coriaceous ketone.
The perch that sea horse is HPA axis not only adjusts centre, and it is the sensitive area that should stimulate injury, exciting sea horse can be restrained should stimulate revulsive coriaceous ketone to secrete, this kind of action is guide by GR place interpose [1] . Sea horse is the cerebrum area with GR most content, in should stimulate cause inside source sex candy is removing complex adjustment in the process with coriaceous elevatory hormone action [2] . The report points out: Chronic society should be stimulated or manacle the expression that should stimulate underground of can different level to move GRmRNA of area of sea horse CA3, sea horse GR MRNA and salt can increase apparently after fighting depressed medicine to treat the expression that coriaceous hormone gets body MRNA, can prevent the expression that should stimulate revulsive CA3 area GRmRNA to reduce [3] . Study the result makes clear originally: In should stimulate a condition to fall, the expression of GRmRNA of area of CA3 of asthmatic bandicoot sea horse decreases apparently; The expression of GR MRNA of area of CA3 of sea horse of bandicoot of group of lung of scanty liver manage and asthma should be stimulated the group is more apparent and elevatory, and expression of GRmRNA of area of CA3 of sea horse of bandicoot of manage lung group and asthma should be stimulated group do not have apparent difference quite, show lung of scanty liver manage to just can answer through adjusting stimulate response, reduce level of serous and coriaceous ketone, the expression of GRmRNA of area of CA3 of elevatory sea horse, thereby correspondence stimulates a condition to issued function of axis of asthmatic bandicoot HPA to remove certain adjustment mussily action.
Bronchus asthma belongs to a doctor of traditional Chinese medicine " roar card " , " asthma card " category. " does element ask? Talk when dirty air standard " the cloud: "Hectic person, cough asthma goes against backache of gas, shoulder, sweat to go out... " show this disease is ill in lung. Bright red Dan Xi thinks: "Roar advocate phlegmy. " " is disease treated because of arteries and veins? Roar is ill " the cloud: "Of roar disease because of, phlegmy drink leaves hot season, form set pattern, conceal at inside, dietary injury or outside the cold that makes sometimes bundle flesh watch, asthmatic disease is made. " clear acting Li Zhongzi the cloud: "Because of inside the energy of life that has clogged up, there is the feeling when blame outside, what midriff has glue to originally is phlegmy, 3 person close, shut refus to be enraged, strike phonic, hair for asthma. " " all pathogeny await theory " the cloud: "Sip cough person... mental disorder photograph is attacked, move along with cough breath... " this shows, phlegmy drink is the crucial factor that asthma comes on. Archaic cure home realises already phlegmy the relation with the seven emotional factors. " card treats collect fill " the cloud: "Terrified care is thought of, phlegmy give birth to Yan therefore. " sluggish of gloomy of diseases with such symptoms as coastal pain, break Yu Shu discharge, the horizontal stroke captures lienal land, lienal lose be good at use and be born inside phlegmy chaotic; Liver gloomy turns internal heat, refine fluid is become phlegmy, this all is born because of gloomy phlegmy. Gloomy phlegmy lay aside at lung, lung of stop up block is angry, do not get announce to fall, and hair cough, card of asthma, roar. Demonstrative affection annals is maladjusted can bring about be born inside phlegmy drink, cause asthma thereby. " golden deficient summary? Phlegmy drink cough is ill " put forward " ill phlegmy drink person, should mix with lukewarm medicine " . Lung of scanty liver manage just is mixed by small Qing Longshang 4 go against medicinal powder composition. The hemp in small Qing Longshang yellow, cassia twig is sudoriferous induce sweat, in order to solve " outside have the feeling when blame " ; Lukewarm lung of dry ginger, the root of Chinese wild ginger, the tuber of pinellia turns water, in order to divide " what the glue in midriff originallies is phlegmy " , hold <> concurrently aid hemp induce sweat of yellow, cassia twig, can arrange at the same time " inside the energy of life that has clogged up " ; Gas of collect of the fruit of Chinese magnoliavine, peony raises beneficial of blood, licorice to enrage in mixing, 3 medicine each other deserves to be able to make the laborious dry of all medicine, in case too over- . 8 flavour are suitable, make water of chill solution, water goes, lung enrages answer easy, announce to fall have right. 4 go against the basic Fang Zhi that coming loose just is recuperation air plane one, square in Gan Wenyi enrages broil licorice with be good at lienal, the root of Chinese thorowax appears unhealthy environmental influences that cause disease, scanty liver to solve gloomy, the gas below trifoliate orange fact breaks a tie, peony beneficial shade raises hematic soft liver. Trifoliate orange fact, the root of Chinese thorowax is suitable, one litre falls, enhance the result of scanty liver regulating the flow of vital energy and removing obstruction to it; The root of Chinese thorowax, peony is suitable, come loose one collect, scanty liver manage is lienal and do not hurt shade; Pleasant of oxalic acid of pleasant of peony, broil turns back, soft liver emergency. 4 medicine each other matchs, make unhealthy environmental influences that cause disease goes free of the Yu Jie, attune that enrage blood. Latter-day pharmacological considers to make clear: 4 go against medicinal powder square in glucoside has the black of active part the root of Chinese thorowax of the root of Chinese thorowax restrain central nervous the action of excited He Kangyan; The serous adrenocorticotro that after antagonism should be stimulated, total glucoside of root of herbaceous peony can lift, can promote lienal lymphocyte hyperplasia at the same time, enhance immune function; Trifoliate orange is solid but scanty liver regulating the flow of vital energy and removing obstruction to it, still can restrain phlogistic sex cell to release histamine [4] . The active ingredient naphtha of trifoliate orange fact also has inhibition to central nervous system [5] . The report thinks: Liver gloomy shows the change of function of system of nerve endocrine immunity more, if patient of liver gloomy card goes,lubricious amine of adrenalin, 5 hydroxide mixes armour much cling to the central nervous such as amine is given qualitative, multinomial index all produces adrenal coriaceous function and immune system change [6] , closely related the network of nerve endocrine immunity of the function that shows liver of doctor of traditional Chinese medicine and modern medicine [7] . The adjustment that liver arouses decisive action in heart behoove is medium, tone liver is had adjust the function with maladjusted function of nerve endocrine immunity [8] .
Head issueing grave and margin system are significant mood activity integrated centre, issueing CRH of excretive of grave head PVN among them is the person that all sorts of answering that basically adjust what stimulate response [9, 10] . Coriaceous hormone is opposite the candy that should surge a level the CRH nerve of PVN of head issueing grave yuan the effect that has negative feedback to adjust, this one action basically uprights by the sea horse place of margin system guide [11, 12] . Study the result makes clear originally: In should stimulate a condition to fall, electropositive nerve of CRH of the nucleus other ventricle of the grave below asthmatic bandicoot yuan increase apparently, the specification is manacled should stimulate can bring about asthmatic bandicoot plasma coriaceous ketone is elevatory, to issueing the inhibition of grave head PVN the gender suffers feedback adjustment effect abate, the CRH electropositive nerve of PVN of head of the grave below bandicoot of group of lung of scanty liver manage yuan should stimulate with asthma the group is more apparent decrease, and manage lung group and asthma should be stimulated group do not have apparent difference quite, explain square medicine of lung of scanty liver manage should stimulate response through adjusting, the CRH electropositive nerve that can reduce PVN of head issueing grave yuan, correspondence stimulates a condition to issue the functional hyperfunction of axis of asthmatic bandicoot HPA to have certain inhibition.
The candy that should surge a level is coriaceous hormone causes loss to central nervous system, since of this kind of injury should stimulate the result of reaction, also be the reason that a lot of afterwards send response. Sea horse is the cerebrum area with GR most content, because this also is the target area that coriaceous hormone atttacks the candy when should be being stimulated optionally, the candy that should surge a level is coriaceous the long-term effect of hormone, will bring about sea horse nerve yuan atrophic, die even [13 ～ 15] . This kind of injury basically is behaved in area of sea horse CA3, this area nerve yuan because lack calcium to combine albumen be caused by,be to easy sensibility of injury [16] . Study the result makes clear originally: In should stimulate a condition to fall, configuration of cell of cone of area of CA3 of asthmatic bandicoot sea horse is irregular below smooth lens, nucleolus solid shrinks, have more dark nerve yuan appear; Configuration of nucleolus of cone of area of CA3 of horse of go into business of transmission report lens is irregular, nuclear different chromatin is apparent grow in quantity, gather agglomerate, afterbirth oar is loose, dilate of body of most line bead, occurrence vacuole, Ji is arranged disorder, nerve of demonstrative sea horse yuan got injure. Area of CA3 of sea horse of bandicoot of group of lung of scanty liver manage behaves the solid that it is a nucleus to shrink below smooth lens nerve yuan decrease; Visible afterbirth oar is loose below electric lens, line bead put oneself in another‘s position is substantial but compact, should stimulate with asthma group compare nerve yuan injury is reduced somewhat. Nerve of cone of area of CA3 of sea horse of bandicoot of manage lung group yuan configuration and asthma should be stimulated group quite, without apparent difference. This shows, lung of scanty liver manage just can be passed adjust should stimulate response, reduce level of serous and coriaceous ketone, reduce nerve of sea horse cone yuan injury, enhance correspondence to stimulate a condition to leave the negative feedback inhibition of head of the grave below asthmatic bandicoot.
When should be being stimulated, coriaceous hormone regards candy as depressor of a kind of immunity, certain effect produces in the immune function that should stimulate a condition to issue pair of airframe. T cell is the active cell that shares airframe cell immunity, CD4 ＋ / CD8+ ratio is producing main effect in maintaining the cellular immunity with normal human body. CD4+T cell can be divided for Th1 and Th2, coriaceous hormone can cause the candy that should surge a level outside the change that the Th1/Th2 inside monocyte balances week blood, make Th2 cell is secreted increase [17] . At present asthmatic incidence of a disease still lifts continuously, with affection stimulation and current life condition are concerned. Be in heart behoove for a long time to stimulate coriaceous ketone can be brought about below condition excessive secrete, affect immune function, be opposite especially the influence of Th1/Th2, increased human body to be opposite asthmatic easy sensibility [18] . This experimental result makes clear: In should stimulate a condition to fall, asthmatic group bandicoot and regular set are compared, serous IL4 is apparently elevatory, IFN γ is reduced apparently; Group of lung of scanty liver manage and bandicoot of manage lung group and asthma should be stimulated group quite, serous IL4 all is reduced, serous IFN γ all lifts; Group of lung of scanty liver manage and manage lung group are compared, serous IFN γ is apparently elevatory, and serous IL4 does not have apparent difference. Accordingly, lung of scanty liver manage just can be passed adjust should stimulate reaction to lift serous IFN γ is mixed reduce serous IL4, antagonism should stimulate what the condition leaves function of asthmatic bandicoot immunity to check status, enhance airframe the resistance to infection, its immunity enhances action may be to pass curb refine sugar coriaceous hormone and of implementation.
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